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fluorescein conjugated wisteria floribunda agglutinin  (Vector Laboratories)


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    Vector Laboratories fluorescein conjugated wisteria floribunda agglutinin
    Fluorescein Conjugated Wisteria Floribunda Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescein+conjugated+wfa/Fluorescein+labeled+Wisteria+Floribunda+Lectin+(WFA%2C+WFL)/bio_rxiv__64898__2026__03__25__714174-7-42-47
    Average 95 stars, based on 147 article reviews
    fluorescein conjugated wisteria floribunda agglutinin - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Neonatal hypoxia-ischemia alters the events governing the hippocampal critical period of postnatal synaptic plasticity leading to deficits in working memory in mice
    Article Snippet: The used subtype and species-specific immunoglobulin were as follows: 1) rabbit IgG isotype (Vector Laboratories, Inc.; Burlingame, CA, USA; RRID: AB_2336355); 2) mouse IgG isotype (Vector Laboratories, Inc.; AB_2336354) and 3) chicken IgY isotype (R&D Systems; RRID: AB_354263). .. Sections were washed in 1× PBS for 10 min, blocked with 10 % NGS in PBS at RT for 90 min, and incubated in green fluorescein conjugated - WFA (Vector Labs, FL-1351-2; at 1:400) in 4 % NGS in PBS for 6 h at RT. ..

    Article Title: Intrauterine Growth Restriction Disrupts the Postnatal Critical Period of Synaptic Plasticity in the Mouse Dorsal Hippocampus in a Model of Hypertensive Disease of Pregnancy.
    Article Snippet: .. Wisteria floribunda Lectin Fluorescent Staining for PNN Detection Fifty-micrometer-thick sections were washed in 1X PBS for 10 min, blocked with 10% NGS in PBS at RT for 90 min, and incubated in green fluorescein conjugated-WFA (Vector Labs, FL1351-2; at 1:400) in 4% NGS in PBS for 6 h at RT. ..

    Article Title: Neonatal hypoxia-ischemia alters the events governing the hippocampal critical period of postnatal synaptic plasticity leading to deficits in working memory in mice.
    Article Snippet: .. Sections were washed in 1X PBS for 10 min, blocked with 10% NGS in PBS at RT for 90 min, and incubated in green fluorescein conjugated - WFA (Vector Labs, FL-1351-2; at 1:400) in 4% NGS in PBS for 6h at RT. ..

    Incubation:

    Article Title: Neonatal hypoxia-ischemia alters the events governing the hippocampal critical period of postnatal synaptic plasticity leading to deficits in working memory in mice
    Article Snippet: The used subtype and species-specific immunoglobulin were as follows: 1) rabbit IgG isotype (Vector Laboratories, Inc.; Burlingame, CA, USA; RRID: AB_2336355); 2) mouse IgG isotype (Vector Laboratories, Inc.; AB_2336354) and 3) chicken IgY isotype (R&D Systems; RRID: AB_354263). .. Sections were washed in 1× PBS for 10 min, blocked with 10 % NGS in PBS at RT for 90 min, and incubated in green fluorescein conjugated - WFA (Vector Labs, FL-1351-2; at 1:400) in 4 % NGS in PBS for 6 h at RT. ..

    Article Title: Cocaine Exposure Modulates Perineuronal Nets and Synaptic Excitability of Fast-Spiking Interneurons in the Medial Prefrontal Cortex
    Article Snippet: After three 10 min washes, tissue was incubated for 2 h with secondary goat antimouse Alexa Fluor-594 antibody (1:500; catalog #R37121, Thermo Fisher Scientific; RRID:AB_2556549). .. After three 10 min washes in PBS, tissue was incubated for 2 h with fluorescein-conjugated WFA (1:500; catalog #FL-1351, Vector Laboratories; RRID:AB_2336875). ..

    Article Title: Cocaine Exposure Modulates Perineuronal Nets and Synaptic Excitability of Fast-Spiking Interneurons in the Medial Prefrontal Cortex
    Article Snippet: After three 10 min washes, tissue was incubated for 2 h with secondary goat anti-mouse Alexa Fluor-594 antibody (1:500; catalog #R37121, Thermo Fisher Scientific; RRID: AB_2556549 ). .. After three 10 min washes in PBS, tissue was incubated for 2 h with fluorescein-conjugated WFA (1:500; catalog #FL-1351, Vector Laboratories; RRID: AB_2336875 ). ..

    Article Title: Intrauterine Growth Restriction Disrupts the Postnatal Critical Period of Synaptic Plasticity in the Mouse Dorsal Hippocampus in a Model of Hypertensive Disease of Pregnancy.
    Article Snippet: .. Wisteria floribunda Lectin Fluorescent Staining for PNN Detection Fifty-micrometer-thick sections were washed in 1X PBS for 10 min, blocked with 10% NGS in PBS at RT for 90 min, and incubated in green fluorescein conjugated-WFA (Vector Labs, FL1351-2; at 1:400) in 4% NGS in PBS for 6 h at RT. ..

    Article Title: Effects of Complete Spinal Cord Transection in Neonatal and Juvenile Rats on Parvalbumin-Positive Inputs and Perineuronal Net Formation on Chx10-Positive V2a Interneurons
    Article Snippet: A series of sections was incubated in a moist chamber overnight at 4°C with a mixture of (1) mouse monoclonal IgG antibody against Chx10 (1:200, D-11, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and rabbit polyclonal IgG antibody against parvalbumin (1:200, Synaptic Systems, Göttingen, Germany), (2) mouse monoclonal IgG antibody against Chx10 (1:200, D-11, Santa Cruz Biotechnology, Inc.) and mouse monoclonal IgM antibody against CSA (1:200, NU-07-001, clone 2H6, Cosmo Bio Co., Ltd, Tokyo, Japan), or (3) mouse monoclonal IgG antibody against Chx10 (1:200, D-11, Santa Cruz Biotechnology, Inc.) only, diluted with 1% normal donkey serum, 0.2% bovine serum albumin, and 0.1% NaN 3 in 0.025 M phosphate-buffered saline with Tween 20 (PBST). .. The sections were rinsed several times with 0.025 M PBST and then incubated for 2 h at room temperature with a mixture of secondary antibodies: (1) Alexa Fluor 488-conjugated donkey anti-rabbit IgG (10 μg/mL; Jackson ImmunoResearch Laboratories, West Grove, PA, USA), cyanine Cy3-conjugated donkey anti-mouse IgG (10 μg/mL; Jackson ImmunoResearch Laboratories), and NeuroTrace 640/660 (1:100, N21483 , Life Technologies Corporation, OR, USA); (2) Alexa Fluor 488-conjugated donkey anti-mouse IgM (10 μg/mL; Jackson ImmunoResearch Laboratories), cyanine Cy3-conjugated donkey anti-mouse IgG (10 μg/mL), and NeuroTrace 640/660 (1:100); or (3) fluorescein-conjugated WFA (1:100, FL-1351, Vector Laboratories, Inc., Burlingame, CA, USA), cyanine Cy3-conjugated donkey anti-mouse IgG (10 μg/mL), and NeuroTrace 640/660 (1:100), diluted with 1% normal donkey serum, 0.2% bovine serum albumin, and 0.1% NaN 3 in 0.025 M PBST. .. The antibody specificity was verified by incubation with 0.5% normal mouse serum (Jackson ImmunoResearch Laboratories) or 0.5% normal rat serum (Jackson ImmunoResearch Laboratories) instead of the primary antibodies.

    Article Title: Neonatal hypoxia-ischemia alters the events governing the hippocampal critical period of postnatal synaptic plasticity leading to deficits in working memory in mice.
    Article Snippet: .. Sections were washed in 1X PBS for 10 min, blocked with 10% NGS in PBS at RT for 90 min, and incubated in green fluorescein conjugated - WFA (Vector Labs, FL-1351-2; at 1:400) in 4% NGS in PBS for 6h at RT. ..

    Labeling:

    Article Title: Sex and region-specific effects of high fat diet on PNNs in obesity susceptible rats
    Article Snippet: Free floating slices were then rinsed in PBS at room temperature (RT) 3 times for 5 min each, 50% alcohol for 30 min, PBS 3x for 5 min each, and incubated in 3% blocking serum (PBS plus 3% normal goat serum [v/vol], S-1000, Vector Laboratories) for 1 hour (RT). .. Slices were then sequentially labeled using anti-PV antibody (SAB4200545, Sigma Aldrich; 1:1000) followed by staining of PNNs with fluorescein conjugated WFA (Vector Laboratories, Burlingame, CA; 1:500) as previously described [ 42 ]. .. Briefly, slices were incubated overnight in 2% blocking serum containing anti-PV antibody (4°C), rinsed in PBS (3×10min), and then incubated with goat anti-mouse IgG (H+L) secondary antibody (A-11005, ThermoFisher; 1:500) in 2% blocking serum (2 hrs at RT).

    Article Title: Sex and region-specific effects of high fat diet on PNNs in obesity susceptible rats.
    Article Snippet: Free floating slices were then rinsed in PBS at room temperature (RT) 3 times for 5 min each, 50% alcohol for 30 min, PBS 3x for 5 min each, and incubated in 3% blocking serum (PBS plus 3% normal goat serum [v/vol], S-1000, Vector Laboratories) for 1 hour (RT). .. Slices were then sequentially labeled using anti-PV antibody (SAB4200545, Sigma Aldrich; 1:1000) followed by staining of PNNs with fluorescein conjugated WFA (Vector Laboratories, Burlingame, CA; 1:500) as previously described [42]. .. Briefly, slices were incubated overnight in 2% blocking serum containing anti-PV antibody (4°C), rinsed in PBS (3×10min), and then incubated with goat anti-mouse IgG (H+L) secondary antibody (A-11005, ThermoFisher; 1:500) in 2% blocking serum (2 hrs at RT).

    Staining:

    Article Title: Sex and region-specific effects of high fat diet on PNNs in obesity susceptible rats
    Article Snippet: Free floating slices were then rinsed in PBS at room temperature (RT) 3 times for 5 min each, 50% alcohol for 30 min, PBS 3x for 5 min each, and incubated in 3% blocking serum (PBS plus 3% normal goat serum [v/vol], S-1000, Vector Laboratories) for 1 hour (RT). .. Slices were then sequentially labeled using anti-PV antibody (SAB4200545, Sigma Aldrich; 1:1000) followed by staining of PNNs with fluorescein conjugated WFA (Vector Laboratories, Burlingame, CA; 1:500) as previously described [ 42 ]. .. Briefly, slices were incubated overnight in 2% blocking serum containing anti-PV antibody (4°C), rinsed in PBS (3×10min), and then incubated with goat anti-mouse IgG (H+L) secondary antibody (A-11005, ThermoFisher; 1:500) in 2% blocking serum (2 hrs at RT).

    Article Title: Sex and region-specific effects of high fat diet on PNNs in obesity susceptible rats.
    Article Snippet: Free floating slices were then rinsed in PBS at room temperature (RT) 3 times for 5 min each, 50% alcohol for 30 min, PBS 3x for 5 min each, and incubated in 3% blocking serum (PBS plus 3% normal goat serum [v/vol], S-1000, Vector Laboratories) for 1 hour (RT). .. Slices were then sequentially labeled using anti-PV antibody (SAB4200545, Sigma Aldrich; 1:1000) followed by staining of PNNs with fluorescein conjugated WFA (Vector Laboratories, Burlingame, CA; 1:500) as previously described [42]. .. Briefly, slices were incubated overnight in 2% blocking serum containing anti-PV antibody (4°C), rinsed in PBS (3×10min), and then incubated with goat anti-mouse IgG (H+L) secondary antibody (A-11005, ThermoFisher; 1:500) in 2% blocking serum (2 hrs at RT).

    Article Title: Intrauterine Growth Restriction Disrupts the Postnatal Critical Period of Synaptic Plasticity in the Mouse Dorsal Hippocampus in a Model of Hypertensive Disease of Pregnancy.
    Article Snippet: .. Wisteria floribunda Lectin Fluorescent Staining for PNN Detection Fifty-micrometer-thick sections were washed in 1X PBS for 10 min, blocked with 10% NGS in PBS at RT for 90 min, and incubated in green fluorescein conjugated-WFA (Vector Labs, FL1351-2; at 1:400) in 4% NGS in PBS for 6 h at RT. ..



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Daily oscillations of neuronal membrane capacitance

    doi: 10.1016/j.celrep.2024.114744

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Slices were then washed in blocking solution (normal goat serum in phosphate buffer (PBS) for 60 min at room temperature on a gentle rocker and then placed for 6 h at room temperature in fluorescein-conjugated Wisteria Floribunda Lectin (WFA, Vector Laboratories, FL-1351) under gentle rocking.

    Techniques: Plasmid Preparation, Recombinant, Software